Barcelona defender Ronald Araujo suffered thigh damage during the El Clasico victory over Real Madrid, the La Liga giants said on Monday.
The Uruguayan international played the full match as Barca won 2-1 on Sunday to move 12 points clear of their rivals at the top of the table.
Barcelona did not say how long the 24-year-old player will be out of play.
Araujo has completed 22 appearances in all matches for Barca this season.